human adipose sv cells  (ZenBio)

Journal: Cell metabolism

Article Title: Cellular aging contributes to failure of cold-induced beige adipocyte formation in old mice and humans

doi: 10.1016/j.cmet.2016.10.023

Figure Lengend Snippet: (A) Senescence activated (SA) βgal staining of inguinal (IGW) adipose depots from two (Young) and six (Old) month old male mice. Arrow points to SA-βgal positive areas. Scale bar = 4 mm. (B) Expression of senescence genes from two and six month old mice. Data are means ± SEM; n=5 mice/group. *P-value <0.05 Old compared to young cells. (C) Expression of senescence genes from young and old BMI matched human SV cells. **P <0.01 Old compared to young cells. (D-E) Two month and six month old SMA-CreERT2; R26RRFP male mice were administered TM; RFP+ cells were FACS isolated at pulse. Cells were stained for Senescence Activated (SA)-βgal (D) or mRNA expression of senescence genes (E) was analyzed. #P<0.05 old SMA/RFP+ compared to young SMA/RFP+ cells. (F) Two or six month old SMA-CreERT2; R26RRFP TM pulsed male mice were randomized to the cold for seven days. (G) Sections from mice described in (F) were analyzed for RFP and UCP1. DAPI was used to visualize nuclei. Scale bar = 200 μm.

Article Snippet: Human Cell Culture Isolated human adipose SV cells were purchased from ZenBio (Research Triangle Park, North Carolina).

Techniques: Staining, Expressing, Isolation

Journal: Cell metabolism

Article Title: Cellular aging contributes to failure of cold-induced beige adipocyte formation in old mice and humans

doi: 10.1016/j.cmet.2016.10.023

Figure Lengend Snippet: (A-C) Total SV cells were isolated from six month old C57/Bl6 male mice (A, B) or SV cells generated from aged human samples. Cells were treated with vehicle or SB202190 (5 μM) for 15 consecutive days. mRNA of senescence markers was examined. *P <0.01 Old compared to young murine cells. *P <0.05 Old compared to young murine cells. (D-F) Human SV cells were treated with vehicle or SB for 15 consecutive days. Subsequently, SA-βgal staining was performed (E) and positive cells were quantified (F). (G) Cells described in (D) were assessed for beige adipogenesis by Oil Red O staining. (H-K) Six month old TM induced SMA-CreERT2; R26RRFP male mice were administered vehicle or SB (75 μg/mouse/day) for five consecutive days (H). RFP+ cells were counted (I) and FACS isolated and examined for phosphor-p38/MAPK and denoted proteins (I, J) and expression of senescence genes (K). Data are means ±S.E.M; n=4 mice/group. #P<0.03 SB202190 treated RFP+ cells compared to vehicle treated RFP+ cells.

Article Snippet: Human Cell Culture Isolated human adipose SV cells were purchased from ZenBio (Research Triangle Park, North Carolina).

Techniques: Isolation, Generated, Staining, Expressing